pyrG [2020-09-27 17:39:48]

CTP synthase (NH₃, glutamine)

Locus: BSU_37150

Isoelectric point: 5.16

Molecular weight: 59.55 kDa

Protein length: 535 aa

Gene length: 1608 bp

Function: pyrimidine biosynthesis

Product: CTP synthase (NH3, glutamine)

Essential: yes

E.C.: 6.3.4.2

Synonyms: ctrA

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of pyrimidine nucleotides
• Groups of genes → Essential genes

Gene

Coordinates: 3,810,693 → 3,812,300

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-glutamine + UTP --> ADP + CTP + 2 H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• CTP synthase family (single member, according to UniProt)

Domains

• Glutamine amidotransferase type-1 domain (aa 292-534) (according to UniProt)

Structure

• 1VCM (from Thermus thermophilus, 52% identity, 70% similarity) PubMed

Expression and Regulation

Operons

• Genes: rpoE-pyrG
  Description: PubMed

  Regulation

  • the mRNA is processed between rpoE and pyrG by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

• Genes: pyrG
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • RNA switch: termination, in RNA switch

  Regulation

  • repressed by cytidine nucleotides (RNA switch) PubMed

Additional information

• the mRNA is cleaved by RNase III PubMed

References

Reviews

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Original publications

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