pyrG [2020-09-27 17:39:48]

CTP synthase (NH₃, glutamine)

locus: BSU_37150

pI: 5.16

mw: 59.55 kDa

protein length: 535 aa

gene length: 1608 bp

function: pyrimidine biosynthesis

product: CTP synthase (NH3, glutamine)

essential: yes

ec: 6.3.4.2

synonyms: ctrA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.4|Biosynthesis/ acquisition of pyrimidine nucleotides]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 3,810,693 → 3,812,300

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O + L-glutamine + UTP --> ADP + CTP + 2 H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• CTP synthase family (single member, according to UniProt)

[SW|Domains]

• [SW|Glutamine amidotransferase type-1 domain] (aa 292-534) (according to UniProt)

Structure

• [PDB|1VCM] (from ''Thermus thermophilus'', 52% identity, 70% similarity) [Pubmed|15296735]

Expression and Regulation

Operons

• genes: [gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]-[gene|CEE04B9FED63AFEAB912791402F7578E852005D1|pyrG]
  description: [Pubmed|29794222]

  regulation

  • the mRNA is processed between [gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE] and [gene|CEE04B9FED63AFEAB912791402F7578E852005D1|pyrG] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

• genes: [gene|CEE04B9FED63AFEAB912791402F7578E852005D1|pyrG]
  description: [Pubmed|2457578]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15252202], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|RNA switch|RNA switch]: termination, in [regulon|RNA switch|RNA switch]

  regulation

  • repressed by cytidine nucleotides ([SW|RNA switch]) [Pubmed|11544212]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

References

Reviews

• 21334966,18535147

Original publications

• 8206849,17302819,15252202,11544212,1709162,12426364,17158658,2457578,28652344,26883633,29794222,31965171